Analysis of ancient bone DNA: techniques and applications.

نویسندگان

  • E Hagelberg
  • L S Bell
  • T Allen
  • A Boyde
  • S J Jones
  • J B Clegg
چکیده

The analysis of DNA from ancient bone has numerous applications in archaeology and molecular evolution. Significant amounts of genetic information can be recovered from ancient bone: mitochondrial DNA sequences of 800 base pairs have been amplified from a 750-year-old human femur by using the polymerase chain reaction. DNA recovery varies considerably between bone samples and is not dependent on the age of the specimen. We present the results of a study on a small number of bones from a mediaeval and a 17th-century cemetery in Abingdon showing the relation between gross preservation, microscopic preservation and DNA recovery.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Novel Substrates as Sources of Ancient DNA: Prospects and Hurdles

Following the discovery in the late 1980s that hard tissues such as bones and teeth preserve genetic information, the field of ancient DNA analysis has typically concentrated upon these substrates. The onset of high-throughput sequencing, combined with optimized DNA recovery methods, has enabled the analysis of a myriad of ancient species and specimens worldwide, dating back to the Middle Pleis...

متن کامل

Is amino acid racemization a useful tool for screening for ancient DNA in bone?

Many rare and valuable ancient specimens now carry the scars of ancient DNA research, as questions of population genetics and phylogeography require larger sample sets. This fuels the demand for reliable techniques to screen for DNA preservation prior to destructive sampling. Only one such technique has been widely adopted: the extent of aspartic acid racemization (AAR). The kinetics of AAR are...

متن کامل

Introducing Satellite Remote Sensing Systems and its Application in Archaeology Case Study: Behshahr Plain- Mazandaran

Human groups have considered the Behshahr plain of Mazandaran in the past Due to its particular geographical shape, location between the Caspian Sea and mountains, and the existence of some rivers in the region. However, our knowledge of this area is limited to several published surveys and archaeological investigation of its ancient sites. No detailed research has conducted on the formation of...

متن کامل

مروری برتکنیک های توالی یابی D‏NA (نسل اول، نسل دوم و نسل سوم)

Introduction: The DNA sequencing is the most important technique in molecular biology by which the order of the nucleotides can be identified in a piece of DNA. There are several different methods for sequencing the DNA. Now, the DNA sequencing has great importance in the medical diagnostics and other medical fields. Some methods have been invented to speed up and increase the efficiency of the...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Philosophical transactions of the Royal Society of London. Series B, Biological sciences

دوره 333 1268  شماره 

صفحات  -

تاریخ انتشار 1991